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Table 7 Oxidative and epigenetic DNA damage and the activities of repair enzymes in the blood and ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a high methionine (Met) level, challenged withClostridium perfringens—(Experiment 2).

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

ItemIleal tissueBlood
8-OHdG
pg/µL DNA
Methylation
DNA,  %
APE-1 
ng/g
TDG
ng/g
ANPG
ng/g
8-OHdG
pg/mL
Methylation
DNA,  %
APE-1 
ng/mL
TDG
ng/mL
ANPG
ng/mL
Arg90*Infection (−)23.40d13.30b1708.2a983.2a38.43a4.136b29.01177.9a93.444.937
Arg90*Infection (+)79.03ab17.69a1000.9b638.2c20.34b6.773a66.25148.7b91.894.416
Arg100*Infection (−)25.92d13.44b1494.4a840.0ab35.17a5.369ab29.02159.7ab91.285.053
Arg100*Infection (+)88.68a16.21ab1008.9b691.3c21.44b5.131ab39.83136.4b85.374.577
Arg110*Infection (−)38.15c14.52abc959.9b666.9bc19.34b6.493a29.22150.0b97.324.686
Arg110*Infection (+)84.28a13.01bc951.8b661.4c25.55b8.144a74.74159.6ab94.073.999
SEM4.1520.38348.3522.981.3770.2432.8633.1031.3400.102
Arg level,  %
 9051.21b15.501354.6a810.7a29.38a5.454b47.63ab163.392.664.677
 10057.30a14.821251.6a765.6a28.30a5.250b34.43b148.188.324.815
 11061.22a13.77955.9b664.2b22.44b7.319a51.98a154.895.704.343
C. perfringens infection1
 –29.16b13.75b1387.5a830.0a30.98a5.333b29.08b162.5a94.014.892a
 +84.00a15.64a987.2b663.6b22.44b6.683a60.27a148.3b90.444.331b
P value
 Arg0.0010.0790.0010.0010.0060.0010.0010.0710.0790.116
 Infection0.0010.0040.0010.0010.0010.0010.0010.0100.1780.004
 Arg × infection0.0010.0010.0010.0010.0010.0030.0010.0090.7890.889
  1. Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg90, Arg100 and Arg110, respectively) and a high level of Met (45% dietary Lys).
  2. 1At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 108 CFU/mL of bacteria. a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).