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Table 7 Oxidative and epigenetic DNA damage and the activities of repair enzymes in the blood and ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a high methionine (Met) level, challenged withClostridium perfringens—(Experiment 2).

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Item

Ileal tissue

Blood

8-OHdG

pg/µL DNA

Methylation

DNA,  %

APE-1 

ng/g

TDG

ng/g

ANPG

ng/g

8-OHdG

pg/mL

Methylation

DNA,  %

APE-1 

ng/mL

TDG

ng/mL

ANPG

ng/mL

Arg90*Infection (−)

23.40d

13.30b

1708.2a

983.2a

38.43a

4.136b

29.01

177.9a

93.44

4.937

Arg90*Infection (+)

79.03ab

17.69a

1000.9b

638.2c

20.34b

6.773a

66.25

148.7b

91.89

4.416

Arg100*Infection (−)

25.92d

13.44b

1494.4a

840.0ab

35.17a

5.369ab

29.02

159.7ab

91.28

5.053

Arg100*Infection (+)

88.68a

16.21ab

1008.9b

691.3c

21.44b

5.131ab

39.83

136.4b

85.37

4.577

Arg110*Infection (−)

38.15c

14.52abc

959.9b

666.9bc

19.34b

6.493a

29.22

150.0b

97.32

4.686

Arg110*Infection (+)

84.28a

13.01bc

951.8b

661.4c

25.55b

8.144a

74.74

159.6ab

94.07

3.999

SEM

4.152

0.383

48.35

22.98

1.377

0.243

2.863

3.103

1.340

0.102

Arg level,  %

 90

51.21b

15.50

1354.6a

810.7a

29.38a

5.454b

47.63ab

163.3

92.66

4.677

 100

57.30a

14.82

1251.6a

765.6a

28.30a

5.250b

34.43b

148.1

88.32

4.815

 110

61.22a

13.77

955.9b

664.2b

22.44b

7.319a

51.98a

154.8

95.70

4.343

C. perfringens infection1

 –

29.16b

13.75b

1387.5a

830.0a

30.98a

5.333b

29.08b

162.5a

94.01

4.892a

 +

84.00a

15.64a

987.2b

663.6b

22.44b

6.683a

60.27a

148.3b

90.44

4.331b

P value

 Arg

0.001

0.079

0.001

0.001

0.006

0.001

0.001

0.071

0.079

0.116

 Infection

0.001

0.004

0.001

0.001

0.001

0.001

0.001

0.010

0.178

0.004

 Arg × infection

0.001

0.001

0.001

0.001

0.001

0.003

0.001

0.009

0.789

0.889

  1. Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg90, Arg100 and Arg110, respectively) and a high level of Met (45% dietary Lys).
  2. 1At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 108 CFU/mL of bacteria. a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).