The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Table 6 **Gene expression in the ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a high methionine (Met) level, challenged withClostridium perfringens—(Experiment 2).**

Item	ZO-1	Occludin	GLP2	OGG1	TFF2
Arg₉₀*Infection (−)	0.794	0.005	0.375	0.184	0.003
Arg₉₀*Infection (+)	1.918	0.022	1.145	0.666	0.011
Arg₁₀₀*Infection (−)	0.841	0.006	0.385	0.172	0.003
Arg₁₀₀*Infection (+)	2.485	0.014	1.590	0.545	0.009
Arg₁₁₀*Infection (−)	0.838	0.005	0.368	0.283	0.003
Arg₁₁₀*Infection (+)	2.253	0.014	1.219	0.616	0.007
SEM	0.120	0.001	0.091	0.044	0.0007
Arg level, %
90	1.356	0.013	0.760	0.425	0.007
100	1.663	0.010	0.987	0.359	0.006
110	1.545	0.009	0.793	0.450	0.005
C. perfringens infection¹
–	0.824^b	0.005^b	0.376^b	0.213^b	0.003^b
+	2.219^a	0.017^a	1.318^a	0.609^a	0.009^a
P value
Arg	0.134	0.144	0.259	0.532	0.421
infection	0.001	0.001	0.001	0.001	0.001
Arg × infection	0.237	0.078	0.301	0.654	0.283

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg₉₀, Arg₁₀₀ and Arg₁₁₀, respectively) and a high level of Met (45% dietary Lys).
¹At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 10⁸ CFU/mL of bacteria. ^a,bMeans within a row with different superscripts differ significantly (P < 0.05).

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