The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Ognik, Katarzyna; Konieczka, Paweł; Mikulski, Dariusz; Jankowski, Jan

doi:10.1186/s13567-020-00776-y

Veterinary Research

Table 5 **Metabolic parameters in the blood of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a low methionine (Met) level, challenged withClostridium perfringens—(Experiment 1).**

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Item	CK U/L	ALT U/L	AST U/L	SOD U/gHb	GPx U/gHb	TP g/L	ALB g/L	UA mmol/L	UREA mmol/L	TC mmol/L	TG mmol/L	MDA µmol/L	GLU mmol/L
Arg₉₀*Infection (−)	0.255	9.15^b	250.1^c	1029.4	14.23^bc	39.61^ab	16.69^b	0.424^ab	1.019	4.069	0.676	1.905^a	9.45^c
Arg₉₀*Infection (+)	0.233	10.52^b	261.4^bc	825.5	17.06^b	41.02^a	18.60^a	0.341^bc	0.789	3.794	0.461	1.886^ab	11.59^ab
Arg₁₀₀*Infection (−)	0.264	12.57^a	300.0^a	1024.8	15.17^bc	28.57^c	13.50^c	0.408^ab	1.171	3.532	0.533	2.014^a	11.78^a
Arg₁₀₀*Infection (+)	0.264	12.71^a	279.1^ab	745.2	31.91^a	34.73^b	18.89^a	0.371^abc	0.942	3.406	0.354	2.089^a	10.02^bc
Arg₁₁₀*Infection (−)	0.275	14.43^a	301.1^a	1082.6	15.30^bc	38.07^ab	18.41^a	0.447^a	1.299	3.250	0.836	1.395^b	11.83^a
Arg₁₁₀*Infection (+)	0.266	6.43^c	248.3^c	1364.5	13.07^c	36.14^ab	19.51^a	0.295^c	1.095	3.410	0.688	2.209^a	9.80^c
SEM	0.006	0.468	4.067	57.66	0.999	0.742	0.325	0.011	0.062	0.070	0.025	0.059	0.215
Arg level, %
90	0.244	9.83^b	255.8^b	927.5^b	15.65^b	40.32^a	17.65^b	0.382	0.904	3.931^a	0.568^b	1.896	10.52
100	0.264	12.64^a	289.5^a	885.0^c	23.54^a	31.65^b	16.19^c	0.390	1.057	3.469^b	0.444^c	2.051	10.90
110	0.271	10.43^ab	274.7^ab	1223.6^a	14.18^b	37.10^ab	18.96^a	0.371	1.197	3.330^b	0.762^a	1.802	10.81
C. perfringens infection¹
–	0.149	12.05^a	283.7^a	1045.6	14.90^b	35.42	16.20^b	0.426^a	1.163	3.617	0.682^a	1.77^b	11.02
+	0.254	9.89^b	262.9^b	978.4	20.68^a	37.30	19.00^a	0.336^b	0.942	3.537	0.501^b	2.06^a	10.47
P value
Arg	0.149	0.001	0.001	0.024	0.001	0.001	0.001	0.638	0.158	0.001	0.001	0.121	0.626
Infection	0.372	0.001	0.001	0.528	0.001	0.054	0.001	0.001	0.077	0.518	0.001	0.005	0.104
Arg × infection	0.734	0.001	0.001	0.074	0.001	0.005	0.001	0.020	0.995	0.348	0.451	0.002	0.000

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg₉₀, Arg₁₀₀ and Arg₁₁₀, respectively) and a low level of Met (30% dietary Lys).
¹At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 10⁸ CFU/mL of bacteria. ^a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).

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