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Table 4 Oxidative and epigenetic DNA damage and the activities of repair enzymes in the blood and ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a low methionine (Met) level, challenged withClostridium perfringens—(Experiment 1).

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

ItemIleal tissueBlood
8-OHdG
pg/µL DNA
Methylation
DNA,  %
APE-1 
ng/g
TDG
ng/g
ANPG
ng/g
8-OHdG
pg/mL
Methylation
DNA,  %
APE-1 
ng/mL
TDG
ng/mL
ANPG
ng/mL
Arg90*Infection (−)24.26d14.92c1731.0a1056.939.014.424c28.66160.183.684.197
Arg90*Infection (+)53.44b18.72ab933.2c596.317.786.825a58.23146.783.994.361
Arg100*Infection (−)28.04d15.37bc1746.2a994.542.415.061bc30.14182.5100.304.747
Arg100*Infection (+)86.21a19.55a995.3c658.921.076.031ab46.46152.292.314.601
Arg110*Infection (−)38.08c19.00ab1382.5b876.337.385.711abc30.20147.494.874.772
Arg110*Infection (+)84.96a13.21c1018.2c651.023.166.017ab52.06144.893.374.978
SEM3.7570.49357.8033.851.6730.1752.2523.0251.6070.075
Arg level, %
 9038.85b16.821332.1826.628.395.62443.44153.4ab83.83b4.279b
 10057.12a17.461370.7826.731.745.54638.30167.4a96.31a4.674ab
 11059.96a16.111200.3763.630.275.86441.13146.1b94.12a4.875a
C. perfringens infection1
 –30.13b16.431619.9a975.9a39.60a5.065b29.67b163.3a92.954.572
 +74.43a17.16982.2b635.4b20.67b6.291a52.25a147.9b89.894.647
P value
 Arg0.0010.3290.0750.4330.3560.6410.3990.0040.0020.003
 Infection0.0010.3270.0010.0010.0010.0010.0010.0030.2920.589
 Arg × infection0.0010.0010.0120.1190.2220.0140.2210.0840.4680.523
  1. Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg90, Arg100 and Arg110, respectively) and a low level of Met (30% dietary Lys).
  2. 1At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 108 CFU/mL of bacteria. a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).