Veterinary Research

Table 4 Oxidative and epigenetic DNA damage and the activities of repair enzymes in the blood and ileal tissues of 42-day-old turkeys fed diets with different arginine (Arg) to lysine (Lys) ratios and a low methionine (Met) level, challenged withClostridium perfringens—(Experiment 1).

From: The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Item	Ileal tissue					Blood
Item	8-OHdG pg/µL DNA	Methylation DNA, %	APE-1 ng/g	TDG ng/g	ANPG ng/g	8-OHdG pg/mL	Methylation DNA, %	APE-1 ng/mL	TDG ng/mL	ANPG ng/mL
Arg₉₀*Infection (−)	24.26^d	14.92^c	1731.0^a	1056.9	39.01	4.424^c	28.66	160.1	83.68	4.197
Arg₉₀*Infection (+)	53.44^b	18.72^ab	933.2^c	596.3	17.78	6.825^a	58.23	146.7	83.99	4.361
Arg₁₀₀*Infection (−)	28.04^d	15.37^bc	1746.2^a	994.5	42.41	5.061^bc	30.14	182.5	100.30	4.747
Arg₁₀₀*Infection (+)	86.21^a	19.55^a	995.3^c	658.9	21.07	6.031^ab	46.46	152.2	92.31	4.601
Arg₁₁₀*Infection (−)	38.08^c	19.00^ab	1382.5^b	876.3	37.38	5.711^abc	30.20	147.4	94.87	4.772
Arg₁₁₀*Infection (+)	84.96^a	13.21^c	1018.2^c	651.0	23.16	6.017^ab	52.06	144.8	93.37	4.978
SEM	3.757	0.493	57.80	33.85	1.673	0.175	2.252	3.025	1.607	0.075
Arg level, %
90	38.85^b	16.82	1332.1	826.6	28.39	5.624	43.44	153.4^ab	83.83^b	4.279^b
100	57.12^a	17.46	1370.7	826.7	31.74	5.546	38.30	167.4^a	96.31^a	4.674^ab
110	59.96^a	16.11	1200.3	763.6	30.27	5.864	41.13	146.1^b	94.12^a	4.875^a
C. perfringens infection¹
–	30.13^b	16.43	1619.9^a	975.9^a	39.60^a	5.065^b	29.67^b	163.3^a	92.95	4.572
+	74.43^a	17.16	982.2^b	635.4^b	20.67^b	6.291^a	52.25^a	147.9^b	89.89	4.647
P value
Arg	0.001	0.329	0.075	0.433	0.356	0.641	0.399	0.004	0.002	0.003
Infection	0.001	0.327	0.001	0.001	0.001	0.001	0.001	0.003	0.292	0.589
Arg × infection	0.001	0.001	0.012	0.119	0.222	0.014	0.221	0.084	0.468	0.523

Diets contained three levels of Arg relative to Lys (90, 100 and 110%; Arg₉₀, Arg₁₀₀ and Arg₁₁₀, respectively) and a low level of Met (30% dietary Lys).
¹At 34, 36 and 37 days of age, birds were infected with 1 mL (per os directly into the crop) of a culture medium of C. perfringens type A strain 56 containing approximately 10⁸ CFU/mL of bacteria. ^a,b,cMeans within a row with different superscripts differ significantly (P < 0.05).

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