The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

Table 2 Genes and primers used in the study.

Gene	Primer	Sequence (5′-3′)	Melting temperature (°C)	Product size (nt)	GenBank access no.
ACTB	Forward	TACCCCATTGAACACGGCAT	58	96	NM_001303173
ACTB	Reverse	CTCCTCAGGGGCTACTCTCA		96	NM_001303173
GAPDH	Forward	AGGATACACAGAGGACCAGGTTG	58	71	NM_001303179
GAPDH	Reverse	CCGCATCAAAGGTGGAGGAATG		71	NM_001303179
ZO-1	Forward	AGAGGCAACTGAACCATAG	58	114	XM_019619275
ZO-1	Reverse	CTGCTGAGAGGCTAATACAA		114	XM_019619275
GLP2	Forward	GCAGTGAAGGAGAAGTGA	58	200	XM_010721176
GLP2	Reverse	GAGGCTGTAAGAAGTAGGA		200	XM_010721176
OCLN	Forward	GCAGATGTCCAGCAGTTA	55	127	XM_019610822
OCLN	Reverse	GTTCACACTCACCTCCTG		127	XM_019610822
TFF2	Forward	AAAATAGCAGCCAGGGAGCG	58	92	XM_010724393
	Reverse	ACTGACGCATTGAAGCAGCA
CLDN15	Forward	GCAAGGAGGCTTCTGAAA	55	161	XM_019618905
	Reverse	CAGTAACTATGTGGCAAGGT
OGG1	Forward	GGGACAAATGGGCACCTG	58	102	XM_010718590
	Reverse	GCAGAGGCAATAGGCTCAG

ACTB: β-actin, GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase, ZO-1: Zonula occludens-1, GLP2: Glucagon-like peptide-2, OCLN: Occludin, TFF2: Trefoil Factor 2, CLDN15: Claudin 15, and OGG1: 8-Oxoguanine glycosylase.

ISSN: 1297-9716