Figure 6

GM RNA induction of IL-1β expression via NLRP3/caspase-1 inflammasomes.A Activation of NLRP3 after GM RNA transfection and GM NDV infection. B Activation of caspase-1 after GM RNA transfection and GM NDV infection. C NLRP3 and IL-1β expression after overexpression of NLRP3 and stimulation with GM RNA. D NLRP3 and IL-1β expression after transfection with Si-NLRP3 and stimulation with GM RNA. DF1 cells were transfected with the NLRP3 overexpression plasmid or Si-NLRP3 for 24 h and then stimulated with GM RNA for 24 h. Samples were collected for subsequent testing. E Caspase-1 activation after incubation with the caspase-1-specific inhibitor Ac-YVAD-CHO and transfection with GM RNA. F IL-1β expression after incubation with the caspase-1-specific inhibitor Ac-YVAD-CHO and transfection with GM RNA. DF1 cells were incubated with Ac-YVAD-CHO (20 μM) for 1 h and then transfected with GM RNA for 24 h. Samples were collected for subsequent testing. *P < 0.05, **P < 0.01 between the experimental and control groups.of illness, the expression levels of IL-1β.