Skip to main content

Table 1 Gene targets and primer sequences used for qPCR

From: Maternal and fetal thyroid dysfunction following porcine reproductive and respiratory syndrome virus2 infection

 

Official symbol

Gene ID

Forward primer

Reverse primer

Annealing temp (°C)

Amplicon length (bp)

House keeping

HPRT1

397351

5′-GGACTTGAATCATGTTTGTG-3′

5′-CAGATGTTTCCAAACTCAAC-3′

61

91

IPO8

100511694

5′-AGGACAGTGGCAGAAGCAAA-3′

5′-TTCAGTTGTTGGTGGGCATA-3′

60

112

RPL19

396989

5′-AACTCCCGTCAGCAGATCC-3′

5′-AGTACCCTTCCGCTTACCG-3′

60

147

STX5

100628048

5′-TGCAGAGTCGTCAGAATGGA-3′

5′-CCAGGATTGTCAGCTTCTCC-3′

60

144

YWHAZ

780440

5′-TGATGATAAGAAAGGGATTGTGG-3′

5′-GTTCAGCAATGGCTTCATCA-3′

62

203

Thyroid hormone reception

DIO2

414379

5′-CTCGGTCATTCTCCTCAAGC-3′

5′-TCACCTGTTTGTAGGCATCG-3′

61

140

DIO3

414378

5′-CCTATCTGCGTGTCTGACGA-3′

5′-GCCTGCTTGAAGAAATCCAG-3′

61

92

ITGAV

397285

5′-GCAACAGTGAAGCCTTAGCA-3′

5′-GCACACTGAAACGAAGACCA-3′

61

132

ITGB3

397063

5′-AATGGGACACAGCCAACAAT-3′

5′-CCACAATCCTGGGACAGTCT-3′

61

126

SLC16A10

100513770

5′-CACCCATTGCAGGGTTACTC-3′

5′-TATGGAGCCAAGGGATGAAA-3′

61

117

SLC16A2

100513513

5′-AGTGGAGTTCCAAGCAGCAT-3′

5′-AGCCCAAACGATCAGTGAAT-3′

61

95

THRA

397387

5′-GAGGAGAACAGTGCCAGGTC-3′

5′-CGACACACTGCTCGTCTTTG-3′

61

121

THRB

396776

5′-AAGGCTGCAAGGGTTTCTTT-3′

5′-TGGCACTGATTTCTGGTGAC-3′

61

112

Cell cycle progression

CCND1

100738589

5′-TGTGCCACAGACGTGAAGTT-3′

5′-GGTGGTAGGACAGGAAGCTG-3′

61

118

CDK1

100155762

5′-CAGCTCGCTACTCAACTCCA-3′

5′-GAGTGCCCAAAGCTCTGAAA-3′

61

135

CDK2

100154715

5′-CGGAGCTTGTTATCGCAAAT-3′

5′-AGGGGTAGGGTTCACAAAGG-3′

61

143

CDK4

100144492

5′-TGGTTACAAGTGGTGGGACA-3′

5′-CCACAGAAGAGAGGCTTTCG-3′

61

208

CDKN1A

100152215

5′-CATGTGGACCTGTTGCTGTC-3′

5′-TTAGGGCTTCCTCTTGGAGA-3′

61

168

Cardiac development and function

AGT

100157073

5′-GCACTTCCAAGGAAAGGTGA-3′

5′-CGACACTGAGGTGGTGTTGT-3′

60

82

ATP2A2

396875

5′-AACGCCCTCAACAGTTTGTC-3′

5′-AGCCACTGGGTCAAATTCAG-3′

61

173

CAMK2D

397674

5′-CAGTACCCATCAAGCCATCC-3′

5′-TGCATGAAGAGGAGGAGAGG-3′

60

197

MYH6

100736765

5′-CTTCGGGAAATTCATCAGGA-3′

5′-GCTCTGGCTTCTTGTTGGAC-3′

62

161

MYH7

396860

5′-ATCCACCCAAGTTCGACAAG-3′

5′-AGATCATCCAGGAGGCGTAG-3′

62

105

PLN

397421

5′-ACCATTGAAATGCCTCAACA-3′

5′-CGATGATGCAAATCAGCAAG-3′

61

97

RYR2

396856

5′-GTGGATACCAGCCAGATCGT-3′

5′-CATACTGCCAGCCAAGTTCA-3′

60

115