Figure 5
Fetal gene expression associated with regulation of cell cycle progression. Expression of genes responsible for promoting cell cycle progression (CCND1, CDK1, CDK2 and CDK4) and their inhibitor (CDKN1A) in the heart (A) and brain (B) tissue of fetuses derived from gestational day matched, non-inoculated control gilts (CON n = 10 heart and 12 brain) and from PRRSV2 challenged dams at 12 days post-inoculation and classified based on serum and thymic viral load as uninfected (UNIF n = 13 heart and 12 brain), high viral load viable (HV-VIA n = 8 heart and 12 brain) or high viral load meconium stained (HV-MEC n = 16 heart and 12 brain). Gene expression was determined by qPCR and fold changes were calculated relative to the average of the CON with unique superscripts denoting statistical differences (P < 0.05) within a given tissue and gene.