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Table 3 Correlation between the 16S rDNA amplicon sequencing and either Ct classes or culture

From: Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction

16S rDNA amplicon sequencing results

B. bronchiseptica

M. cynos

qPCR results

Culture results

qPCR results

r

P-value

r

P-value

r

P-value

Phyla

 Proteobacteria

0.54

0.012

0.70

0.0022

  

 Tenericutes

    

0.66

0.0018

Families

 Alcaligenaceae

0.66

0.0015

0.70

0.0021

  

 Mycoplasmataceae

    

0.66

0.0018

Genera

 Bordetella

0.73

< 0.001

0.70

0.0022

  

 Mycoplasma

    

0.66

0.0018

Species

 Bordetella_bronchiseptica

0.71

< 0.001

0.70

0.0022

  

 Mycoplasma_cynos

    

0.73

< 0.001

 Bordetella_Otu00473

0.70

< 0.001

0.68

0.0017

  
  1. Significant positive correlation results between the bacterial composition of the LM in all dogs (n = 24) for B. bronchiseptica and M. cynos at each taxonomic level and either B. bronchiseptica and M. cynos Ct classes or the culture. qPCR, quantitative polymerase chain reaction; r, Spearman correlation coefficient