GtxA is a virulence factor that promotes a Th2-like response during Gallibacterium anatis infection in laying hens

Table 2 List of the primers used in RT-qPCR analysis of mRNA expression of selected host genes

Name	Primer	Primer sequence(5′–3′)	Accession	Conc. (mM)
bcl-2	Forward	GATGACCGAGTACCTGAACC	NM205339	0.2
bcl-2	Reverse	CAGGAGAAATCGAACAAAGGC	NM205339	0.2
bax	Forward	TCCTCATCGCCATGCTCAT	XM422067	0.4
bax	Reverse	CCTTGGTCTGGAAGCAGAAGA	XM422067	0.4
caspase-8	Forward	TGGCCCTCTTGAACTGAAAG	AY057940	0.4
caspase-8	Reverse	TCCACTGTCTGCTTCAATACC	AY057940	0.4
caspase-9	Forward	CGAAGGAGCAAGCACGACAG	AY057940	0.2
caspase-9	Reverse	CCGCAGCCCTCATCTAGCAT	AY057940	0.2
caspase-3	Forward	TGGCCCTCTTGAACTGAAAG	AY057940	0.4
caspase-3	Reverse	TCCACTGTCTGCTTCAATACC	AY057940	0.4
IL-6	Forward	GCTCGCCGGCTTCGA	AJ250838	0.2
IL-6	Reverse	GGTAGGTCTGAAAGGCGAACAG	AJ250838	0.2
IL-4	Forward	AACATGCGTCAGCTCCTGAAT	AJ621735	0.4
IL-4	Reverse	TCTGCTAGGAACTTCTCCATTGAA	AJ621735	0.4
IL-10	Forward	CATGCTGCTGGGCCTGAA	J621614	0.4
IL-10	Reverse	CGTCTCCTTGATCTGCTTGATG	J621614	0.4
TNF-α	Forward	GCCCTTCCTGTAACCAGATG	NM_204267	0.2
TNF-α	Reverse	ACACGACAGCCAAGTCAACG	NM_204267	0.2
β-actin	Forward	CCGCTCTATGAAGGCTACGC	L08165	0.4
β-actin	Reverse	CTCTCGGCTGTGGTGGTGAA	L08165	0.4

ISSN: 1297-9716