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Figure 7 | Veterinary Research

Figure 7

From: Intranasally administered protein coated chitosan nanoparticles encapsulating influenza H9N2 HA2 and M2e mRNA molecules elicit protective immunity against avian influenza viruses in chickens

Figure 7

Protective efficacy of the CNP-based HA2-HA1 + M2e vaccine against LPAI viruses. Chickens (n = 17 in each group) were intranasally vaccinated with empty CNPs, CNPs delivering antigens in both protein (Pr) and mRNA formats (CNPs + mRNA + Pr) or CNPs delivering antigens in protein (Pr) format only (CNPs + Pr) and 3 weeks later chickens were boasted with the same dose and route. At 28 days post-first vaccination, all the vaccinated chickens were challenged with 104 TCID50 of either H7N9 or H9N2 virus. The protective efficacy was determined by the estimation of viral RNA copy numbers in the cloacal swab samples of the immunized chickens (n = 5) after the challenge with the virulent AIV. A Protection efficacy against the H9N2 challenge. B Protection efficacy against the H9N2 challenge. **p < 0.01. ***p < 0.001. ns, non-significant. a, with respect to CNPs only; b, significant with respect to CNPs and CNPs + Pr.

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Veterinary Research

ISSN: 1297-9716