Figure 9
From: Effect of apo-lactoferrin on leukotoxin and outer membrane vesicles of Mannheimia haemolytica A2
Lkt fromM. haemolyticaA2 grown in medium supplemented with apo- and holo-BLf is active. A Ovine macrophages were treated with purified Lkt secreted by M. haemolytica A2 grown in medium supplemented with apo- and holo-BLf (2, 4, 6 and 8 µM) or purified Lkt plus RPMI with the same concentration of apo- and holo-Lf (2, 4, 6 and 8 apo- plus apo-Lf or 2, 4, 6 and 8 holo- plus holo-Lf). B Ovine macrophages were treated with OMVs released by M. haemolytica A2 grown in medium supplemented with apo- and holo-BLf (2 or 8 µM).