Figure 5

PCV2 down-regulates tight junction protein levels in STEC. STEC were uninfected or infected with PCV2 for 24 h, 36 h and 48 h. A Protein levels of ZO-1 and occludin were determined by Western blotting and band intensities relative to control were analyzed. B mRNA levels of ZO-1 and occludin were analyzed with qRT-PCR. The results are described as mean ± SD of three independent experiments. Significant differences were determined using the Student t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant.