Figure 4

A. muciniphilaregulates the proliferation of intestinal stem cells though Wnt/β-catenin signaling pathway. A–F The fold induction of relative mRNA expressions of Wnt3, Axin2, Lgr5, Lrp5, TCF3 and GS3Kβ in chicks’ colon; n = 12. G, H Confocal images (β-catenin staining, green; and DAPI staining, blue) of colon, more than 20 crypts were recorded. The β-catenin fluorescence intensity was detected. Scale bars 200 μm.