Table 2 Primers used for plasmid construction
Primer | Sequence (5′–3′) | Usage |
|---|---|---|
CMV-ISG15-F | CGGAATTCATGGGTAGGGAACTGAAGGTGAAGA | Amplification of ISG15 |
CMV-ISG15-R | CGGGATCCGCACTCGGTGGGGTGCTCCC | |
3.1-ISG15AA-A2 | TGGCCCTGTCCCCGCCGCCCGCAGGCGCAG | Amplification of ISG15 mutant |
3.1-ISG15AA-B1 | CTGCGCCTGCGGGCGGCGGGGACAGGGCCA | |
Flag-ISG15-F | CGGAATTCTATGGGTAGGGAACTGAAGGTGAAGA | Amplification of ISG15 |
Flag-ISG15-R | CGGGATCCGCACTCGGTGGGGTGCTCC | |
Myc-BECN1-F | CGGAATTCTATGGAGGGGTCTAAGACATCCA | Amplification of BECN1 |
Myc-BECN1-R | CGGGATCCTTTGTTATAAAACTGTGAGGATACCC | |
Flag-BECN1-F | CGGAATTCCATGGAGGGGTCTAAGACATCCA | Amplification of BECN1 |
Flag-BECN1-R | CGGGATCCTTTGTTATAAAACTGTGAGGATACCC | |
Flag-ISG15-BECN1-A2 | CTACAGAATCTGGGGAGGTAGCCCCCCCGCAGGCGCAGATT | Amplification of ISG15-BECN1 |
Flag-ISG15-BECN1-B1 | ATCTGCGCCTGCGGGGGGGCATGGAGGGGTCTAAGACATCCA | |
Myc-HERC5-F | TGC TCTAGA ATGGAGCGGAGACCACGGAG | Amplification of HERC5 |
Myc-HERC5-R | CG GGATCC GCCAAATCCCCTGTGGTTGC | |
GST-BECN1-F | CGGAATTCATGGAGGGGTCTAAGACATCCA | Amplification of BECN1 |
GST-BECN1-R | CGGGATCCTTTGTTATAAAACTGTGAGGATACCC | |
GST-HERC5-F | CG GGATCC ATGGAGCGGAGACCACGGAG | Amplification of HERC5 |
GST-HERC5-R | CCG CTCGAG TCAGCCAAATCCCCTGTGGTT |