Table 1 Primers used for RT-qPCR
Primer | Sequence (5′–3′) | Usage |
|---|---|---|
ISG15-F | AGGGAACTGAAGGTGAAGATG | RT-qPCR for detection of ISG15 |
ISG15-R | CAGACGCTGCTGGAAGG | |
MX1-F | TCTGTAAGCAGGAGACCATCAACT | RT-qPCR for detection of MX1 |
MX1-R | TTTCTCGCCACGTCCACTATC | |
β-actin-F | CAAGGACCTCTACGCCAACAC | RT-qPCR for detection of β-actin |
β-actin-R | TGGAGGCGCGATGATCTT | |
CSFV-F | GAGAAGGACAGCAGAACTAAGC | RT-qPCR for detection of CSFV |
CSFV-R | TTACCGCCCATGCCAATAGG |