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Table 1 Primers used for RT-qPCR

From: Antiviral activity of ISG15 against classical swine fever virus replication in porcine alveolar macrophages via inhibition of autophagy by ISGylating BECN1

PrimerSequence (5′–3′)Usage
ISG15-FAGGGAACTGAAGGTGAAGATGRT-qPCR for detection of ISG15
ISG15-RCAGACGCTGCTGGAAGG 
MX1-FTCTGTAAGCAGGAGACCATCAACTRT-qPCR for detection of MX1
MX1-RTTTCTCGCCACGTCCACTATC 
β-actin-FCAAGGACCTCTACGCCAACACRT-qPCR for detection of β-actin
β-actin-RTGGAGGCGCGATGATCTT 
CSFV-FGAGAAGGACAGCAGAACTAAGCRT-qPCR for detection of CSFV
CSFV-RTTACCGCCCATGCCAATAGG