Figure 3

ISG15 expression is upregulated in CSFV-infected PAMs.A, B Expression of ISG15 in PAMs incubated with CSFV (MOI = 1) at 0, 3, 6, 12, 24, 36, and 48 hpi. AISG15 expression was quantified by RT-qPCR. and Western blot. C, D Expression of ISG15 in PAMs incubated with CSFV (MOI = 0.1, 1, and 3). The expression of ISG15 was quantified by RT-qPCR (C) and Western blot (D) at 24 hpi. β-actin served as an internal control. Data (A, C) represent the mean ± SD of three independent experiments and were measured in technical duplicate. Comparisons between groups were calculated using Student’s t-test. **P < 0.01; ***P < 0.001