Figure 2

ISG15 is induced by IFN-α and contributes to IFN antiviral activity against CSFV. PAMs were treated with different amounts of IFN-α (0, 1, 10, and 100 ng/mL) for 24 h. AISG15 expression was determined by RT-qPCR. B Cell viability was examined by MTT assay. C Mock- and IFN-α-treated (10 ng/mL) PAMs were harvested at the indicated times post-treatment. ISG15 expression was quantified by RT-qPCR. IFN-α (10 ng/mL) was added as a positive control. ShISG15 and shN cells were mock-treated or treated with IFN-α (10 ng/mL) for 24 h prior to CSFV infection (MOI = 1). D CSFV genomic RNA was quantified by RT-qPCR. E ISG15 mRNA and protein levels were analyzed by RT-qPCR and Western blot. Data (A, B, C, D, and E) represent the mean ± SD of three independent experiments and were measured in technical duplicate. Comparisons between groups were calculated using Student’s t-test. ***P < 0.001