Increased CD163 expression upon PRRSV-1 Lena inoculation. Quantification of total CD163+ cells from the mock, LV and Lena-inoculated groups. NC: mock-inoculated group. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc test (*p < 0.05, **p < 0.01). All data are expressed as mean value of three experiments ± SD. Cells used in this experiment are from the same group used for cell characterization (Figure 5).