Figure 7

PRRSV-1 Lena subtype 3 replicates better than LV subtype 1 in nasal surface macrophages. Primary nasal cells isolated after 48 h and 72 h digestion were inoculated with LV and Lena. A Cells were co-immunostained for PRRSV N-protein (red) and CD163 (green) at 12 hpi. Scale bar: 25 µm. Small boxes in the IF pictures represent zoomed pictures of the infected CD163⁺ cells indicated by arrows. B Identification and quantification of PRRSV-1 LV and Lena-infected cells. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc test (***p < 0.001). All data are expressed as mean value of three experiments ± SD. All inoculated cells are from the same group used for cell characterization (Figure 5).