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Figure 4 | Veterinary Research

Figure 4

From: Isolation and characterization of a new population of nasal surface macrophages and their susceptibility to PRRSV-1 subtype 1 (LV) and subtype 3 (Lena)

Figure 4

Schematic representation of the whole nose digestion system. The top of the figure shows the frontal and sagittal planes of the pig nasal cavity where the tubes (turquoise blue) were inserted. The digestion was performed for 72 h in total. Enzyme mix with dissociated cells was collected and refreshed by a new enzyme mix every 24 h to isolate cells from the lamina propria. Small holes (represented by blue spots) were made at the end of the silicone tubes for flushing the enzyme mix efficiently. The whole nose digestion was performed on ice for a mild enzyme reaction. Cells collected at 24, 48, and 72 h were directly used for cell characterization and susceptibility to PRRSV-1 infection.

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