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Figure 6 | Veterinary Research

Figure 6

From: Lung endothelial cells are sensitive to epsilon toxin from Clostridium perfringens

Figure 6

Etx oligomerizes on 1G11 mouse endothelial cells. MDCK (A) and 1G11 (B) cells were treated with 50 nM of GFP-pEtx or GFP-Etx for different incubation times (lane 1: non-treated cells; lane 2: cells treated with GFP-pEtx for 120 min exposure at 37 °C; lanes 3 to 6: cells treated with GFP-Etx for 15, 30, 60, and 120 min exposure at 37 °C, respectively; lane 7: GFP-pEtx recombinant protein; lane 8: GFP-Etx recombinant protein; and lane 9: GFP recombinant protein). Note the oligomer complex formation above 250 kDa (arrows, A and B) in both MDCK and 1G11 cells. See the GFP-pEtx around 60 kDa (black arrowhead, A and B), the GFP-Etx around 56 kDa (gray arrowhead, A and B), and the GFP around 27 kDa (asterisk, A and B). α-tubulin was used as a loading control.

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