Figure 6
From: Lung endothelial cells are sensitive to epsilon toxin from Clostridium perfringens
Etx oligomerizes on 1G11 mouse endothelial cells. MDCK (A) and 1G11 (B) cells were treated with 50 nM of GFP-pEtx or GFP-Etx for different incubation times (lane 1: non-treated cells; lane 2: cells treated with GFP-pEtx for 120 min exposure at 37 °C; lanes 3 to 6: cells treated with GFP-Etx for 15, 30, 60, and 120 min exposure at 37 °C, respectively; lane 7: GFP-pEtx recombinant protein; lane 8: GFP-Etx recombinant protein; and lane 9: GFP recombinant protein). Note the oligomer complex formation above 250 kDa (arrows, A and B) in both MDCK and 1G11 cells. See the GFP-pEtx around 60 kDa (black arrowhead, A and B), the GFP-Etx around 56 kDa (gray arrowhead, A and B), and the GFP around 27 kDa (asterisk, A and B). α-tubulin was used as a loading control.