Figure 3
From: Lung endothelial cells are sensitive to epsilon toxin from Clostridium perfringens
Etx produces morphological changes in 1G11 mouse endothelial cells. MDCK (A–C), 1G11 (D–F) and NIH/3T3 cells (G–I) were treated with 50 nM GFP (A, D and G), GFP-pEtx (B, E and H) or GFP-Etx (C, F and I) for 120 min at 37 °C. In MDCK and 1G11 cells, cortical actin was disorganized, changing its location from the plasma membrane to the cytosol (arrowheads in C and arrows in F, respectively). Note 1G11 cells with a more rounded morphology in GFP-Etx (F) compared to the elongated morphology in GFP or GFP-pEtx treatments (D and E, respectively). No changes were detected in NIH/3T3 cells. Bar 30 µm.