Figure 1From: Lung endothelial cells are sensitive to epsilon toxin from Clostridium perfringensEtx binds to the plasma membrane of 1G11 mouse endothelial cells. Confocal microscopy images show the binding of GFP-Etx to the plasma membrane of MDCK cells, as a positive control (A–C) but not to HeLa cells, as a negative control (D–F). GFP-Etx also bound to the plasma membrane of the 1G11 cells (G–I), quite like MDCK cells. The cells were incubated with GFP-pEtx, and nuclei were stained with TO-PRO3 (Blue). Note GFP-pEtx binding to the plasma membrane of MDCK cells (arrows, C) and 1G11 cells (arrows, I). Scale bar 20 µm.Back to article page