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Figure 2 | Veterinary Research

Figure 2

From: Acetylation at K108 of the NS1 protein is important for the replication and virulence of influenza virus

Figure 2

Growth kinetics and NS1 expression levels of WSN-wt, WSN-NS1-108R, and WSN-NS1-108Q in MDCK and A549 cells. Monolayers of MDCK (A) and A549 (B) cells were infected with different MOIs of each virus, and the supernatant samples were collected at the indicated time points. The virus titers were measured in MDCK cells. Each data point indicates the mean ± SEM of three independent experiments, ***P < 0.001, *P < 0.05. Western blotting was conducted to measure the NS1 expression levels of the three viruses in MDCK (C) and A549 (D) cells, and the relative quantification of NS1 was determined by ImageJ software (version 1.49).

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