Table 1 Primers for RT-qPCR and plasmid construction
Primers | Sequence (5′–3′) |
|---|---|
pig GAPDH-Forward | CCTTCCGTGTCCCTACTGCCAAC |
pig GAPDH-Reverse | GACGCCTGCTTCACCACCTTCT |
pig IFN-β-Forward | TGCAACCACCACAATTCC |
pig IFN-β-Reverse | CTGAGAATGCCGAAGATCTG |
pig IFN-α-Forward | GCCTCCTGCACCAGTTCTACA |
pig IFN-α-Reverse | TGCATGACACAGGCTTCCA |
poSn-Forward | CGTGTTGTGGCCTCTTCTCT |
poSn-Reverse | CACGTTGCAAGTCAGGTTGG |
pig DAP12-Forward | ACCCGGAAACAACACATCGC |
pig DAP12-Reverse | TACTGCCTCTGTGTGTTGAGG |
PRRSV-ORF7-Forward | AAACCAGTCCAGAGGCAAGG |
PRRSV-ORF7-Reverse | GCAAACTAAACTCCACAGTGTAA |
poSn-infusion-Forward | CTGGCTAGTTAAGCTATGGACTTCCTGCTCCTGCT |
poSn-infusion-Reverse | GCCCTCTAGACTCGAGGACTGTGCTTTTCACAGA |
poSn-ECD-infusion-Reverse | GCCCTCTAGACTCGAGGTGCTGGAACAGATGCA |
poSn-TCD-Forward | CCAAGCTTGCCACCATGCTTCTCTGGTTCCT |
poSn-TCD-Reverse | CCGCTCGAGGACTGTGCTTTTCACAGACTG |
DAP12-Forward | CCCAAGCTTGCCACCATGGGAAGACTGGGGCCAT |
DAP12-XhoI-Reverse | CCGCTCGAGTCACTTGTAGTACTGCCGCTGGGTA |
DAP12-ΔICD-Forward | CGGAATTCTCAGAGAGAATGCAGCTGCT |
DAP12-ΔICD-Reverse | GCTCTAGATCACACAGCCAGGGCGAT |
DAP12-ΔECD-Forward | CGGAATTCTGGCATCCTGGCG |
DAP12-ΔECD-Reverse | GCTCTAGATTTGTAATACTGCCTCTGTGTGTTG |
DAP12(D50A)-Forward | GGGGATCTGGTGCTGGCCCTCCTCATCGCCCTG |
DAP12(D50A)-Reverse | CAGGGCGATGAGGAGGGCCAGCACCAGATCCCC |
DAP12-ΔTM1-Forward | GCTGCTCCGCCGTGAGCCCCCTGGGGGATCTGGTGCTGAC |
DAP12-ΔTM1-Reverse | GTCAGCACCAGATCCCCCAGGGGGCTCACGGCGGAGCAGC |
DAP12-ΔTM2-Forward | GCATCCTGGCGGGGATCGTGCTCCTCATCGCCCTGGCTGT |
DAP12-ΔTM2-Reverse | ACAGCCAGGGCGATGAGGAGCACGATCCCCGCCAGGATGC |
DAP12-ΔTM3-Forward | TGGGGGATCTGGTGCTGACCTACTCCCTGGGTCGGCTGGT |
DAP12-ΔTM3-Reverse | ACCAGCCGACCCAGGGAGTAGGTCAGCACCAGATCCCCCA |