Figure 7
poSn-DAP12 pathway mediates the inhibition of type I IFN production in response to poly (I:C).A PAMs were transfected with sipoSn-332# or sipoSn-1983# for 36 h, and then stimulated with 0.25 μg/mL poly (I:C) for 12 h. RT-qPCR analysis was performed to measure the mRNA abundance of poSn and IFN-α/β. B, C CRL-2843-CD163 cells with poSn overexpression were transfected with 2.5 μg/mL poly (I:C) for the indicated time periods (0, 2, 4 h). poSn expression was determined by IB (B). IFN-β transcription was examined by RT-qPCR (C). D, EDAP12 knockdown PAMs were stimulated with 2 μg/mL poly (I:C) for the indicated time periods (0, 3, 6 h). DAP12 knockdown was determined by IB (D). IFN-α/β transcription was detected by RT-qPCR (E). Quantification data were indicated as mean ± SEM. Statistical significances were shown by Student t test: *p < 0.05, **p <0.01, ***p <0.001.