Figure 5
poSn-DAP12 pathway facilitates PRRSV infection through suppressing type I IFN production.A–D PAMs were transfected with siDAP12-433# for 36 h, and then infected with PRRSV (MOI = 1) for the indicated time periods (4, 12, 24, 48 h). DAP12 knockdown was determined by RT-qPCR and IB A. PRRSV ORF7 was examined by RT-qPCR at 4 or 12 h post-infection B. TCID50 assay was performed to measure the viral titers at 24 or 48 h post-infection C. RT-qPCR was used to detect transcription of IFN-α/β at 4 or 12 h post-infection D, E Pig IFN-β promoter was activated by 3×Flag-pRIG-I overexpression, and the relative luciferase activity (Rel. luc. Act) of pig IFN-β promoter was measured in CRL-2843-CD163 cells transfected with poSn-myc-his and/or 3×Flag-DAP12 by dual-luciferase reporter assays. Data were indicated as mean ± SEM. Statistical significances were shown by Student t test: *p < 0.05, **p <0.01, ***p <0.001.