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Figure 4 | Veterinary Research

Figure 4

From: Porcine sialoadhesin suppresses type I interferon production to support porcine reproductive and respiratory syndrome virus infection

Figure 4

Residues 51–57 are responsible for interaction between DAP12 and poSn.A HEK-293T cells were co-transfected with 6 μg poSn-TCD-myc-his and 6 μg 3×Flag-DAP12 or DAP12-D50A for 48 h. Co-IP experiments were performed with anti-Flag mAb or anti-myc mAb. The indicated proteins were analyzed by IB from eluted proteins and WCLs. B HEK-293T cells were co-transfected with 6 μg poSn-TCD-myc-his and 6 μg 3×Flag-DAP12 or the indicated DAP12 mutants (DAP12-ΔTM1, ΔTM2 or ΔTM3) for 48 h. WCLs were incubated with Protein A/G beads bound to anti-Flag or anti-myc mAb at 4 °C overnight. IB analysis was conducted for detecting DAP12, DAP12 mutants or poSn-TCD. IB panels were representative from three independent experiments.

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Veterinary Research

ISSN: 1297-9716