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Figure 3 | Veterinary Research

Figure 3

From: Porcine sialoadhesin suppresses type I interferon production to support porcine reproductive and respiratory syndrome virus infection

Figure 3

poSn-TCD and DAP12 TMD are responsible for their interaction.A HEK-293T cells were transfected with 3×Flag-DAP12 (6 μg) and poSn-TCD-myc-his (6 μg) or poSn-ECD-myc-his ( μg) for 48 h. WCLs were subjected to IP assays with anti-myc mAb at 4 °C for 3 h. IB was used to detect the indicated proteins. B IP assays with anti-myc mAb were performed using WCLs from HEK-293T cells co-transfected with poSn-myc-his (10 μg) and 6 μg 3×Flag-DAP12-ΔICD or 3×Flag-DAP12-ΔECD for 48 h. IB analysis was performed to examine the specific proteins. IB panels were representative from three independent experiments.

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Veterinary Research

ISSN: 1297-9716