Figure 1

poSn promotes PRRSV infection by inhibiting PRRSV-triggered IFN-βtranscription.A–D PAMs were pre-inoculated with PRRSV (MOI = 1) at 37 °C for 1 h. After washing with serum-free RPMI-1640, PAMs were transfected with sipoSn-332# or sipoSn-1983# for 47 h. poSn knockdown was determined by FCM A. IB was used to detect the abundance of PRRSV N protein B. PRRSV TCID₅₀/mL of supernatants was measured, which was independently repeated three times C. IFN-β transcription was detected by RT-qPCR, which was independently repeated three times D. Data were denoted as mean ± SEM. Statistical analysis was applied to Student t test: *p < 0.05, ***p < 0.001. FCM images were representative from two independent experiments.