Figure 8
duHMGB1 mediates anti-viral activity. A duHMB1 overexpression induced inhibition of viral replication for NDRV, DTMUV and DPV. The duHMGB1 vector (pcDNA3.0-duHMGB1-Flag) and the empty vector (EV, control group) were transfected for 24 h. Then cells were infected with viruses at a dose of 10 TCID50/mL; B duHMGB1 knocking-down induced enhanced replication of NDRV. Si-duHMGB1-2 and Si-NC (control group) were transfected in DEF cells for 36 h. Then cells were infected with NDRV at 1 TCID50/mL. The culture supernatants (same sentence for A and B) were collected for detecting the viral titers at 12, 24, 36 and 48 hpi by RT-qPCR. Viral copy number was expressed as copy number (log10) per µL RNA or DNA related to the virus. Mann–Whitney U test was performed to evaluate the differences. *P < 0.05; **P < 0.01; ***P < 0.001.