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Figure 7 | Veterinary Research

Figure 7

From: High-mobility group box 1 protein (HMGB1) from Cherry Valley duck mediates signaling pathways and antiviral activity

Figure 7

Overexpression of duHMGB1 activates the IFN-I signaling pathway. A dual luciferase reporter gene assay was used to study the IFN-I signaling pathway. pcDNA3.0(+)-duHMGB1-Flag and empty vector (control group) plasmids (500 ng/well) were co-transfected with reporter plasmids (100 ng/well) (A) pGL3-IRF7; (B) PGL3-IFN-β with pRL-TK (normalization) (50 ng/well). After 36 hpt, cells were harvested, and luciferase activity was measured. Relative IRF-7-, or IFN-β-reporter activation was calculated as fold-change in normalized Firefly luciferase activity with reference to mean control values set to 1. Data were means from three independent experiments and each experiment was analyzed in triplicate. Mann–Whitney U test was performed to evaluate the differences. *P < 0.05; **P < 0.01; ***P < 0.001.

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