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Figure 4 | Veterinary Research

Figure 4

From: High-mobility group box 1 protein (HMGB1) from Cherry Valley duck mediates signaling pathways and antiviral activity

Figure 4

LPS stimulation induces transfer of over-expressed recombinant duHMGB1 from nucleus to cytoplasm. Immunofluorescence imaging was performed without LPS stimulation (control group) (A) and after LPS stimulation (B). DEF cells were transfected with pcDNA3.0(+)-duHMGB1-Flag plasmid for 24 h. Indirect immunofluorescence was performed after staining with mouse anti-Flag antibody and FITC-labelled goat anti-mouse IgG (green) 12 h (T1), 24 h (T2) and 36 h (T3) after LPS stimulation. Nuclei were counterstained in blue (DAPI). All lines represent 25 μm length.

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