duHMGB1 is localized in the nucleus under normal condition. A Expression of recombinant pcDNA3.0(+)-duHMGB1 in DEF cells after transfection as shown by western blotting using anti-Flag antibody; B Sub-cellular localization of over-expressed duHMGB1 as shown by immunofluorescence. duHMGB1 plasmid was transfected in DEF cells for 24 h. Indirect immunofluorescence was performed using mouse anti-Flag antibody and FITC-labelled goat anti-mouse IgG (green). Nuclei were counterstained in blue (DAPI). All lines represent 25 μm length.