Figure 1

Characterization of duHMGB1. A Protein motifs of duHMGB1 were analyzed using SMART. B Amino-acid alignment of duHMGB1. Alignment was performed using the Clustal X program and edited with Boxshade. HMGB1 sequences are shown for the Cherry Valley duck (Du), chicken (Ch), human (Hu), and mouse (Mu). Black shading indicates amino acid identity, gray shading indicates similarity (50% threshold). C A phylogenic tree based on duHMGB1 the amino acid sequences of the Cherry Valley duck and other species. The neighbor-joining tree was generated using MEGA 7.0, and a 1000-replicate bootstrap analysis was performed. Scale bar is 0.05. GenBank accession numbers are shown in Additional file 2.