Figure 5

Basal levels of inflammatory mediators in cultured PCLS. Nitrite produced by PCLS (500 μm) prepared from CSF1R-eGFP transgenic chickens was assessed in the supernatant over 8 days of culture by Griess assay (white) (A). The supernatant was harvested every 24 h. As positive control, on day 7 a number of PCLS were stimulated with LPS for 24 h (black) and the supernatant harvested on day 8. n = 6–10 slices generated from 4 individual birds. iNOS (B) and IL-1β (C) mRNA levels were assessed by qPCR in the PCLS over 21 days post slice and normalised to 28S expression (white). n = 6 slices generated from 4 individual birds. iNOS and IL-1β levels were also assessed in a fresh lung biopsy sample (grey) that had not been sliced into sections or cultured with DMEM. n = 3–4 slices generated from 3 individual birds. All data are represented as the mean ± SD. *p < 0.05 all time points compared.