Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Precision cut lung slices: a novel versatile tool to examine host–pathogen interaction in the chicken lung

Figure 4

Immunostaining of PCLS. PCLS (200–300 μm) prepared from PA12 SPF chickens 1 day post slice were stained with a panel of antibodies and examined by confocal microscopy. Nuclei are represented in blue (DAPI). Actin cytoskeleton staining using rhodamine phalloidin (red) (A). Longitudinal section revealing boundaries of capillaries from the parabronchi (A, panel i); Transversal section revealing a parabronchus and air capillaries in greater details (A, panels ii and iii). The white box (A, panels ii) represents the area digitally enlarged (A, panels iii). von Willebrand Factor staining (red) (B), a marker of endothelial cells. Overall view of vWF-stained endothelial cells within the lung parenchyma (B, panel i); Longitudinal section (B, panel ii) with a schematic drawing of capillary networks beneath the lung parenchyma (dotted lines). The white box (B, panel ii) represents the area digitally enlarged (B, panel iii). Capillary ends are observed scattered in the tissue (B, panel ii); a capillary ramification close to a parabronchus and a capillary end in greater detail (B, panel iii). Longitudinal section showing MCRL1-B+ phagocytes (green) (C) within the lung parenchyma (C, panels i and ii), especially close to the septae. The white box (C, panel i) represents the area digitally enlarged (C, panel ii). Transversal section showing CD45+ leucocytes (green) within the lung parenchyma (D, panels i and ii), scattered in the atria and septae. The white box (D, panel i) represent the area digitally enlarged (D, panel ii). Images were captured using a Leica TCS P8 confocal microscope. Scale bars = 75 μm (B, panel i) and 25 μm. Images are representative of 3 to 4 PCLS randomly selected from 2 birds per staining strategy.

Back to article page