Figure 3

PCLS cytoskeleton structure under different media conditions. PCLS (200–300 μm) prepared from PA12 SPF chickens were cultured in DMEM/F12/FCS (A), DMEM/F12 (B), or DMEM (C) from 1 (i), 3 (ii) or 7 days (iii and iv) post slice. PCLS were fixed and β-tubulin filaments (green) and nuclei (blue) visualized. The white boxes (A, panel iii, B, panels iii and C iii) represent the area digitally enlarged (A, panel iv, B, panel iv, C, panel iv respectively). Images were captured using a Leica TCS P8 confocal microscope at ×630 magnification. Scale bars = 25 μm. Images are representative of 3 to 4 PCLS randomly selected from 2 birds per time point and condition.