Figure 1

PCLS viability over time. PCLS viability was assessed by AlamarBlue assay (A and C) and Live/Dead staining (B). PCLS (500 μm) prepared from CSF1R-eGFP transgenic chickens were cultured for 7 days in either DMEM/F12/FCS (blue circle), DMEM/F12 (red triangle) or DMEM (black square) and viability was assessed using the AlamarBlue assay (A). n = 6–18 slices generated from 3 birds, data are represented as the mean ± SD. PCLS prepared from SPF PA12 chickens (200–300 μm) were cultured for 7 days in either DMEM/F12/FCS (blue circle), DMEM/F12 (red triangle) or DMEM (black square) and were stained with a Live/Dead kit and subsequently examined by epifluorescence microscopy (B). The number of dead cells were enumerated in images from 3 to 4 PCLS generated from 2 birds at different time-points using the Fiji-ImageJ image processing software. Data are expressed as the mean ± SD, per time-point of one representative experiment. n = 3–4 slices. The maintenance of cell viability in PCLS (500 μm) prepared from CSF1R-eGFP transgenic chickens was assessed in DMEM media by AlamarBlue assay (C). n = 16 slices generated from 2 birds, data are represented as the mean ± SD.