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Table 2 Comparison of the diagnostics tests IHC, EIA and RT-QuIC on selected tissues

From: Goats naturally devoid of PrPC are resistant to scrapie

GenotypeGoat#HippocampusSCLNSpleen
IHCaEIAbRT-QuICcIHCaEIAbIHCaEIAb
PRNP+/+4160.52.2863/30.00.0
PRNP+/+4173.0> 3.503/30.00.0
PRNP+/+5291.5> 3.503/30.00.0
PRNP+/+5360.50.5942/30.00.0
PRNP+/Ter4512.5> 3.503/30.00.0
PRNP+/Ter4691.5> 3.503/30.00.0
PRNP+/Ter5272.0> 3.503/30.00.0
PRNP+/Ter5331.5> 3.502/30.00.0
PRNPTer/Ter4130.00/30.00.0
PRNPTer/Ter4570.00/30.00.0
PRNPTer/Ter4760.00/30.00.0
PRNPTer/Ter4900.00/30.00.0
  1. SCLN, superficial cervical lymph node; IHC, immunohistochemistry; EIA, enzyme immunoassay; RT-QuIC, real-time quaking-induced conversion.
  2. aImmunohistochemical score of PrPSc from 0 to 3.
  3. bIDEXX enzyme immunoassay absorbance values (A450). Negative results (−) are below cut off: 0.198. All negative samples were below 0.033.
  4. cRT-QuIC results show positive replicates/total number of replicates.