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Table 2 Comparison of the diagnostics tests IHC, EIA and RT-QuIC on selected tissues

From: Goats naturally devoid of PrPC are resistant to scrapie

Genotype

Goat#

Hippocampus

SCLN

Spleen

IHCa

EIAb

RT-QuICc

IHCa

EIAb

IHCa

EIAb

PRNP+/+

416

0.5

2.286

3/3

0.0

0.0

PRNP+/+

417

3.0

> 3.50

3/3

0.0

0.0

PRNP+/+

529

1.5

> 3.50

3/3

0.0

0.0

PRNP+/+

536

0.5

0.594

2/3

0.0

0.0

PRNP+/Ter

451

2.5

> 3.50

3/3

0.0

0.0

PRNP+/Ter

469

1.5

> 3.50

3/3

0.0

0.0

PRNP+/Ter

527

2.0

> 3.50

3/3

0.0

0.0

PRNP+/Ter

533

1.5

> 3.50

2/3

0.0

0.0

PRNPTer/Ter

413

0.0

0/3

0.0

0.0

PRNPTer/Ter

457

0.0

0/3

0.0

0.0

PRNPTer/Ter

476

0.0

0/3

0.0

0.0

PRNPTer/Ter

490

0.0

0/3

0.0

0.0

  1. SCLN, superficial cervical lymph node; IHC, immunohistochemistry; EIA, enzyme immunoassay; RT-QuIC, real-time quaking-induced conversion.
  2. aImmunohistochemical score of PrPSc from 0 to 3.
  3. bIDEXX enzyme immunoassay absorbance values (A450). Negative results (−) are below cut off: 0.198. All negative samples were below 0.033.
  4. cRT-QuIC results show positive replicates/total number of replicates.