Figure 6

Role of the IL-11/STAT3 signaling pathway in alleviating PEDV-induced apoptosis. A pIL-11 reduced the apoptosis rate of Vero E6 cells after PEDV infection. Vero E6 cells were pretreated with pIL-11 and STAT3 inhibitor, and then mock-infected or infected with PEDV. Cells were harvested at 36 hpi, dually labeled with Annexin V and PI, and subjected to FACS analysis. Images are representative of three independent experiments. B The graph represents the percentage of each quadrant in A. C pIL-11 influenced the transcription of apoptosis-related genes after PEDV infection. qPCR assay of the mRNA levels of apoptosis-resistance genes in Vero E6 cells from different treatment groups. Amplified products were quantitated using the comparative threshold cycle (CT) method, and the results were normalized to the endogenous levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). All results are representative of three independent experiments. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.