Effect of neuraminidase treatment on the replication of rotaviruses in primary enterocytes and MA104 cells. Primary enterocytes and MA104 Cells were treated with 50 mU/mL NA from Vibrio Cholerae for 1 h at 37 °C before inoculation with rotavirus at a MOI of 0.1. The percentage of infection was measured by immunofluorescence staining at 12 hpi. Data are expressed as the mean ± SD of the results of three separate experiments. Statistically significant differences are indicated with two asterisks (p < 0.01) and three asterisks (p < 0.001).