EGTA treatment has no effect on the post-internalization stage of rotavirus particle in enterocytes. Cells were inoculated with rotavirus at 37 °C for 1 h. After 3 washings, cells were treated with EGTA or PBS for 30 min. Then, the cells were further incubated for 9 h at 37 °C. A Representative confocal image of infection in enterocytes. Scale bar: 50 µm. The percentage of infected cells (B) and virus titer (C) were determined. Data are expressed as the mean ± SD of the results of three separate experiment.