Figure 3
From: Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
Rotavirus preferentially infects the basolateral surface of enterocytes and disruption of ICJ overcomes the restriction of rotavirus infection at the apical surface. A Representative confocal images of rotavirus infection (green) in enterocytes. Scale bar: 50 µm. To compare epithelial cell susceptibility to rotavirus, cells were exposed at either the apical surface or the basolateral surface to rotavirus 12R050 (G5P[7]) and 12R046 (G9P[23]). B The total number of infected cells per well was counted for each condition. C The virus titer was determined in supernatant for each condition. Data are expressed as the mean ± SD of the results of three separate experiments. Statistically significant differences are indicated with one asterisk (p < 0.05) and two asterisks (p < 0.01).