Kinetics of rotavirus 12R050 (G5P) replication in enterocytes after treatment with EGTA. A Representative confocal images of rotavirus infection (green) in primary enterocytes after a 30-min treatment with EGTA or PBS at 0, 9, 18 and 72 hpi. Scale bar: 50 µm. B The percentage of rotavirus infected enterocytes was analyzed by immunofluorescence staining. C Virus titer was determined in the supernatant with MA104 cells. Data are expressed as the mean ± SD of the results of three separate experiments. Statistically significant (p < 0.05) differences are indicated with an asterisk.