Figure 9

Western blot analysis of rTsENO and M-rTsENO binding to human PLG. A SDS-PAGE of rTsENO, M-rTsENO, ScENO, soluble antigens of ML and ES antigens of ML. M: protein marker; 1: rTsENO; 2: M-rTsENO; 3: ScENO; 4: soluble antigens of ML; 5: ES antigens of ML. B Western blot analysis of TsENO and M-rTsENO binding to human PLG. M: protein marker; 1: rTsENO; 2: M-rTsENO; 3: ScENO; 4: soluble antigens of ML; 5: ES antigens of ML; 6: BSA. The proteins mentioned above were blotted on a NC membrane and pre-incubated with human PLG. Subsequently, the membrane was incubated with an anti-PLG antibody. ScENO and BSA served as the positive control and negative control, respectively. The bands representing PLG binding to rTsENO (52 kDa), M-rTsENO (52 kDa), ScENO (47 kDa), soluble antigens of ML (26.9–82.9 kDa, including 52 kDa) and ES antigens of ML (52 kDa) were observed on the strips. The band representing PLG binding of rTsENO was more distinct than that of M-rTsENO, although the loaded amount of proteins was consistently 20 µg. There were some other unnamed PLG binding proteins with molecular masses ranging from 26.9 to 82.9 kDa among ML soluble antigens.