Figure 3
From: Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli
Influence of the lsr operon on the bacterial AI-2 internalization curve. A The AI-2 activity of bacterial cell-free culture fluids was monitored by an AI-2 bioassay. APEC94 and APEC94Δlsr(Cm) produced AI-2 at the logarithmic phase, but the AI-2 activity of APEC94Δlsr(Cm) (1864 RLUs) was 2.26-fold higher than the value of APEC94 (823 RLUs) (p < 0.05). B In the AI-2 internalization curve of APEC94, the activity of AI-2 began to decrease rapidly at 4 h and at 8 h the AI-2 activity was nearly negative, while the activity of AI-2 in APEC94Δlsr(Cm) cell suspensions remained high during the 8 h (p < 0.001). All samples were measured in triplicate. p values less than 0.05 were considered significant.