Table 3 Differences in the conditions used for the conventional and improved LuSIAs with CC81-GREMG cells
Parameter | Conventional | Improvement |
|---|---|---|
The number of CC81-GREMG cells | 5 × 104 cells/well | 1 × 104 cells/well |
The number of cells in the milk | 5 × 105, 1 × 105, and 2 × 104 cells/well | 1 × 105 cells/well |
Days of co-culture | 96Â h (4Â days) | 120Â h (5Â days) |
Activation of B-cells by pokeweed mitogen stimulation | None | 1 µg/mL |