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Table 1 Strains and plasmids used in this study

From: Surface-exposed loops L7 and L8 of Haemophilus (Glaesserella) parasuis OmpP2 contribute to the expression of proinflammatory cytokines in porcine alveolar macrophages

Strains or plasmidsDescriptionReferences
Strains
 Escherichia coli DH5αFL80DlacZDM15D (lacZYA-argF) U169 recA1 endA1 hsdR17Laboratory collection
 Haemophilus parasuis  
 SC096Serovar 4 clinical isolate[16]
 ΔompP2SC096 ΔompP2, GmR
 ompP2ΔLoop7SC096 ompP2ΔLoop7, KmRThis study
 ompP2ΔLoop8SC096 ompP2ΔLoop8, KmR
Plasmids
 pMD-19TT-vector, AmpRTakara
 pK18mobsacBSuicide and narrow-broad-host vector, KanR[33]
 pBAD18-KmKan resistance cassette-carrying complement vector, KanR[34]
 ZY01A fragment containing the upstream and downstream sequences of the OmpP2 gene in pK18mobsacB, KanRThis study
 pZY02A fragment containing the upstream and downstream sequences of the Loop7 gene in pK18mobsacB, KanRThis study
 pZY03A fragment containing the upstream and downstream sequences of the Loop8 gene in pK18mobsacB, KanRThis study