Strains or plasmids | Description | References |
---|---|---|
Strains | ||
Escherichia coli DH5α | FL80DlacZDM15D (lacZYA-argF) U169 recA1 endA1 hsdR17 | Laboratory collection |
Haemophilus parasuis | ||
SC096 | Serovar 4 clinical isolate | [16] |
ΔompP2 | SC096 ΔompP2, GmR | |
ompP2ΔLoop7 | SC096 ompP2ΔLoop7, KmR | This study |
ompP2ΔLoop8 | SC096 ompP2ΔLoop8, KmR | |
Plasmids | ||
pMD-19T | T-vector, AmpR | Takara |
pK18mobsacB | Suicide and narrow-broad-host vector, KanR | [33] |
pBAD18-Km | Kan resistance cassette-carrying complement vector, KanR | [34] |
ZY01 | A fragment containing the upstream and downstream sequences of the OmpP2 gene in pK18mobsacB, KanR | This study |
pZY02 | A fragment containing the upstream and downstream sequences of the Loop7 gene in pK18mobsacB, KanR | This study |
pZY03 | A fragment containing the upstream and downstream sequences of the Loop8 gene in pK18mobsacB, KanR | This study |