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Table 1 Strains and plasmids used in this study

From: Surface-exposed loops L7 and L8 of Haemophilus (Glaesserella) parasuis OmpP2 contribute to the expression of proinflammatory cytokines in porcine alveolar macrophages

Strains or plasmids

Description

References

Strains

 Escherichia coli DH5α

FL80DlacZDM15D (lacZYA-argF) U169 recA1 endA1 hsdR17

Laboratory collection

 Haemophilus parasuis

  

 SC096

Serovar 4 clinical isolate

[16]

 ΔompP2

SC096 ΔompP2, GmR

 ompP2ΔLoop7

SC096 ompP2ΔLoop7, KmR

This study

 ompP2ΔLoop8

SC096 ompP2ΔLoop8, KmR

Plasmids

 pMD-19T

T-vector, AmpR

Takara

 pK18mobsacB

Suicide and narrow-broad-host vector, KanR

[33]

 pBAD18-Km

Kan resistance cassette-carrying complement vector, KanR

[34]

 ZY01

A fragment containing the upstream and downstream sequences of the OmpP2 gene in pK18mobsacB, KanR

This study

 pZY02

A fragment containing the upstream and downstream sequences of the Loop7 gene in pK18mobsacB, KanR

This study

 pZY03

A fragment containing the upstream and downstream sequences of the Loop8 gene in pK18mobsacB, KanR

This study